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Lepr -deficient, T2DM mice show an exacerbated IFN beta pulmonary response following SARS-CoV-2 infection. Relative gene expression of Ifna1 (A) , Ifnb1 (B) , and Ifng (C) in the lungs of mock-infected and SARS-CoV-2-infected lean and Lepr -deficient, T2DM mice at 2 dpi and 4 dpi revealed the activation of Ifnb1 , but not Ifna1 and Ifng . (D) Distribution and (E) abundance of Ifnb1 mRNA in the lung of Lepr -deficient, T2DM, and lean mice by <t>RNAscope</t> ® ISH. Ifnb1 mRNA is primarily expressed in the cytoplasm and nucleus of bronchiolar epithelial cells. × 200 total magnification (Scale bar: 200 μm). Bars represent the mean ± standard deviation. ** P ≤ 0.01; *** P ≤ 0.001.
Rnascope ® 2.5 Lsx Reagent Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnascope ® 2.5 lsx reagent kit/product/Advanced Cell Diagnostics Inc
Average 90 stars, based on 1 article reviews
rnascope ® 2.5 lsx reagent kit - by Bioz Stars, 2026-03
90/100 stars
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rnascope® 2.5 lsx reagent kit - red  (Advanced Cell Diagnostics Inc)
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Advanced Cell Diagnostics Inc rnascope® 2.5 lsx reagent kit - red
Lepr -deficient, T2DM mice show an exacerbated IFN beta pulmonary response following SARS-CoV-2 infection. Relative gene expression of Ifna1 (A) , Ifnb1 (B) , and Ifng (C) in the lungs of mock-infected and SARS-CoV-2-infected lean and Lepr -deficient, T2DM mice at 2 dpi and 4 dpi revealed the activation of Ifnb1 , but not Ifna1 and Ifng . (D) Distribution and (E) abundance of Ifnb1 mRNA in the lung of Lepr -deficient, T2DM, and lean mice by <t>RNAscope</t> ® ISH. Ifnb1 mRNA is primarily expressed in the cytoplasm and nucleus of bronchiolar epithelial cells. × 200 total magnification (Scale bar: 200 μm). Bars represent the mean ± standard deviation. ** P ≤ 0.01; *** P ≤ 0.001.
Rnascope® 2.5 Lsx Reagent Kit Red, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnascope® 2.5 lsx reagent kit - red/product/Advanced Cell Diagnostics Inc
Average 90 stars, based on 1 article reviews
rnascope® 2.5 lsx reagent kit - red - by Bioz Stars, 2026-03
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Lepr -deficient, T2DM mice show an exacerbated IFN beta pulmonary response following SARS-CoV-2 infection. Relative gene expression of Ifna1 (A) , Ifnb1 (B) , and Ifng (C) in the lungs of mock-infected and SARS-CoV-2-infected lean and Lepr -deficient, T2DM mice at 2 dpi and 4 dpi revealed the activation of Ifnb1 , but not Ifna1 and Ifng . (D) Distribution and (E) abundance of Ifnb1 mRNA in the lung of Lepr -deficient, T2DM, and lean mice by <t>RNAscope</t> ® ISH. Ifnb1 mRNA is primarily expressed in the cytoplasm and nucleus of bronchiolar epithelial cells. × 200 total magnification (Scale bar: 200 μm). Bars represent the mean ± standard deviation. ** P ≤ 0.01; *** P ≤ 0.001.
Rnascope 2.5 Lsx (Brown) Detection Kit 322700, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnascope 2.5 lsx (brown) detection kit 322700/product/Advanced Cell Diagnostics Inc
Average 90 stars, based on 1 article reviews
rnascope 2.5 lsx (brown) detection kit 322700 - by Bioz Stars, 2026-03
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rnascope® 2.5 lsx reagent kit-brown  (Advanced Cell Diagnostics Inc)
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Lepr -deficient, T2DM mice show an exacerbated IFN beta pulmonary response following SARS-CoV-2 infection. Relative gene expression of Ifna1 (A) , Ifnb1 (B) , and Ifng (C) in the lungs of mock-infected and SARS-CoV-2-infected lean and Lepr -deficient, T2DM mice at 2 dpi and 4 dpi revealed the activation of Ifnb1 , but not Ifna1 and Ifng . (D) Distribution and (E) abundance of Ifnb1 mRNA in the lung of Lepr -deficient, T2DM, and lean mice by <t>RNAscope</t> ® ISH. Ifnb1 mRNA is primarily expressed in the cytoplasm and nucleus of bronchiolar epithelial cells. × 200 total magnification (Scale bar: 200 μm). Bars represent the mean ± standard deviation. ** P ≤ 0.01; *** P ≤ 0.001.
Rnascope® 2.5 Lsx Reagent Kit Brown, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnascope® 2.5 lsx reagent kit-brown/product/Advanced Cell Diagnostics Inc
Average 90 stars, based on 1 article reviews
rnascope® 2.5 lsx reagent kit-brown - by Bioz Stars, 2026-03
90/100 stars
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rnascope 2.5 lsx reagent kit red  (Advanced Cell Diagnostics Inc)
90
Advanced Cell Diagnostics Inc rnascope 2.5 lsx reagent kit red
Lepr -deficient, T2DM mice show an exacerbated IFN beta pulmonary response following SARS-CoV-2 infection. Relative gene expression of Ifna1 (A) , Ifnb1 (B) , and Ifng (C) in the lungs of mock-infected and SARS-CoV-2-infected lean and Lepr -deficient, T2DM mice at 2 dpi and 4 dpi revealed the activation of Ifnb1 , but not Ifna1 and Ifng . (D) Distribution and (E) abundance of Ifnb1 mRNA in the lung of Lepr -deficient, T2DM, and lean mice by <t>RNAscope</t> ® ISH. Ifnb1 mRNA is primarily expressed in the cytoplasm and nucleus of bronchiolar epithelial cells. × 200 total magnification (Scale bar: 200 μm). Bars represent the mean ± standard deviation. ** P ≤ 0.01; *** P ≤ 0.001.
Rnascope 2.5 Lsx Reagent Kit Red, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnascope 2.5 lsx reagent kit red/product/Advanced Cell Diagnostics Inc
Average 90 stars, based on 1 article reviews
rnascope 2.5 lsx reagent kit red - by Bioz Stars, 2026-03
90/100 stars
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rnascope 2.5 lsx reagent kit-brown  (Advanced Cell Diagnostics Inc)
90
Advanced Cell Diagnostics Inc rnascope 2.5 lsx reagent kit-brown
Lepr -deficient, T2DM mice show an exacerbated IFN beta pulmonary response following SARS-CoV-2 infection. Relative gene expression of Ifna1 (A) , Ifnb1 (B) , and Ifng (C) in the lungs of mock-infected and SARS-CoV-2-infected lean and Lepr -deficient, T2DM mice at 2 dpi and 4 dpi revealed the activation of Ifnb1 , but not Ifna1 and Ifng . (D) Distribution and (E) abundance of Ifnb1 mRNA in the lung of Lepr -deficient, T2DM, and lean mice by <t>RNAscope</t> ® ISH. Ifnb1 mRNA is primarily expressed in the cytoplasm and nucleus of bronchiolar epithelial cells. × 200 total magnification (Scale bar: 200 μm). Bars represent the mean ± standard deviation. ** P ≤ 0.01; *** P ≤ 0.001.
Rnascope 2.5 Lsx Reagent Kit Brown, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnascope 2.5 lsx reagent kit-brown/product/Advanced Cell Diagnostics Inc
Average 90 stars, based on 1 article reviews
rnascope 2.5 lsx reagent kit-brown - by Bioz Stars, 2026-03
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Lepr -deficient, T2DM mice show an exacerbated IFN beta pulmonary response following SARS-CoV-2 infection. Relative gene expression of Ifna1 (A) , Ifnb1 (B) , and Ifng (C) in the lungs of mock-infected and SARS-CoV-2-infected lean and Lepr -deficient, T2DM mice at 2 dpi and 4 dpi revealed the activation of Ifnb1 , but not Ifna1 and Ifng . (D) Distribution and (E) abundance of Ifnb1 mRNA in the lung of Lepr -deficient, T2DM, and lean mice by RNAscope ® ISH. Ifnb1 mRNA is primarily expressed in the cytoplasm and nucleus of bronchiolar epithelial cells. × 200 total magnification (Scale bar: 200 μm). Bars represent the mean ± standard deviation. ** P ≤ 0.01; *** P ≤ 0.001.

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Diabetes exacerbates SARS-CoV-2 replication through ineffective pulmonary interferon responses, delayed cell-mediated immunity, and disruption of leptin signaling

doi: 10.3389/fcimb.2025.1513687

Figure Lengend Snippet: Lepr -deficient, T2DM mice show an exacerbated IFN beta pulmonary response following SARS-CoV-2 infection. Relative gene expression of Ifna1 (A) , Ifnb1 (B) , and Ifng (C) in the lungs of mock-infected and SARS-CoV-2-infected lean and Lepr -deficient, T2DM mice at 2 dpi and 4 dpi revealed the activation of Ifnb1 , but not Ifna1 and Ifng . (D) Distribution and (E) abundance of Ifnb1 mRNA in the lung of Lepr -deficient, T2DM, and lean mice by RNAscope ® ISH. Ifnb1 mRNA is primarily expressed in the cytoplasm and nucleus of bronchiolar epithelial cells. × 200 total magnification (Scale bar: 200 μm). Bars represent the mean ± standard deviation. ** P ≤ 0.01; *** P ≤ 0.001.

Article Snippet: The RNAscope ® ISH assay was performed using the RNAscope ® 2.5 LSx reagent kit (Advanced Cell Diagnostics) on the automated BOND RX m platform (Leica Biosystems, Buffalo Grove, IL) as described previously ( ).

Techniques: Infection, Gene Expression, Activation Assay, RNAscope, Standard Deviation